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Based on clinically, well tested, standard formulations
VitriBlast and ThermoBlast vitrification media, are both optimised for blastocysts and are based on well-tested standard formulations (Lane et al). Since the formulations have been used considerably, Nidacon can provide you with a very detailed protocol for vitrifying blastocysts, just for your security.
The main problem when freezing cells is the formation of intracellular ice crystals during both cooling and warming. These ice crystals have a detrimental effect on cell survival. Vitrification, the extremely rapid freezing of cellular material, makes it possible to freeze cells without forming ice crystals within the cells. The result of vitrification is a very homogenous structure, an amorphous crystalline structure, as seen with glass and obsidian, materials which fracture easily, have sharp edges and smooth surfaces (glass transition). The cryoprotectants used for vitrification are a combination of Dimethyl Sulfoxide (DMSO), Ethylene Glycol, Sucrose and Ficoll. Nidacon’s vitrification fluids, VitriBlast for cooling and ThermoBlast for warming/thawing, can both be used with different types of vitrification-devices, but we recommend our closed device.
VitriBlast and ThermoBlast are based on clinically, well tested, standard formulations (Lane et al). Numerous publications demonstrate their effectiveness regarding both survival of blastocysts and pregnancy rates. In addition, follow-up studies have been published on live-births, where a comparison is made with new-births from fresh blastocysts, slow-frozen early cleavage stage embryos and vitrified blastocysts (Wikland M et al.(2010).
Use a legally marketed device indicated for use in blastocyst vitrification procedures. Use a closed system to prevent the potential risk of viral contamination using open systems where the sample comes in direct contact with liquid nitrogen. The device needs to meet the following rate of cooling: minimum 1.800ºC/min (High security straw)
The Fertility Centre at Carlanderska Hospital in Gothenburg, Sweden, has been using this formulation since 2005 with excellent results. Blastocyst survival rates are 5% higher (84% vs 80%), and pregnancy rate per transfer is much higher (54% vs 37%), when compared to the slow-freezing technique for cleavage stage embryos.
Article No. / Name / Volume
VBK-010 / VitriBlast Kit / 3×10 mL (Not available in Europe)
Ahlström A1, Westin C, Wikland M, Hardarson T., (2013)
Human Reproduction, Vol.28, No.5 pp. 1199–1209
Lane M et al, (1999)
Fertility and Sterility., Vol 72, No 6, pp1073-1078
Mukaida T et al. (2003)
Hum Reprod., Vol. 18, No. 2, pp384-391
Takahashi K et al. (2005)
Fertil Steril., Vol. 84, No. 1, 88-92
Wikland M et al. (2010).
Human Reproduction, Vol.00, No.0 pp. 1–9
T. Hardarson et al. (2006)
Acta Obstet Gynecol Scand. 86 pp119-120