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NidaCon International AB

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431 37 Mölndal


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Based on clinically, well tested, standard formulations 

ThermoBlast and VitriBlast are vitrification and thawing media optimised for blastocysts and based on well-tested, standard formulations (Lane et al). Since the formulations have been used considerably, Nidacon can provide you with a very detailed protocol for vitrifying and thawing blastocysts, just for your security.

  • The main problem when freezing cells is the formation of intracellular ice crystals during both cooling and warming/thawing, since these ice crystals have a detrimental effect on cell survival. Vitrification, the extremely rapid freezing of cellular material, makes it possible to freeze cells without forming ice crystals within the cells. The result of vitrification is a very homogeneous structure, an amorphous crystalline structure, as seen with glass and obsidian, materials which fracture easily, have sharp edges and smooth surfaces (glass transition). The cryoprotectants used for vitrification are a combination of Dimethyl Sulfoxide (DMSO), Ethylene Glycol, Sucrose and Ficoll. Nidacon’s VitriBlast for cooling and ThermoBlast for warming/thawing, can be used with different types of vitrification-devices, although we recommend our closed-system device.

  • ThermoBlast and VitriBlast are based on clinically, well tested, standard formulations (Lane et al). Numerous publications demonstrate their effectiveness regarding both survival of blastocysts and pregnancy rates. In addition, follow-up studies have been published on live-births, where a comparison is made with babies from fresh blastocysts, slow-frozen early cleavage stage embryos and vitrified blastocysts (Wikland M et al. 2010).

  • The Fertility Centre at Carlanderska Hospital in Gothenburg, Sweden, has been using this formulation since 2005 with excellent results. Blastocyst survival rates are 5% higher (84% vs 80%), and pregnancy rate per transfer is much higher (54% vs 37%), when compared to the slow-freezing technique for cleavage stage embryos.

    • Sodium chloride
    • Potassium chloride
    • Magnesium sulphate
    • Potassium dihydrogen phosphate
    • Sodium bicarbonate
    • hSA (human Serum Albumin)
    • Purified water
    • Glucose
    • Calcium lactate
    • Sodium pyruvate
    • EDTA
    • HEPES
    • Sucrose

    Performance Characteristics

    • pH: 7.25-7.45.
    • Endotoxin levels: <0.5 EU/mL.
    • MEA: Re-expanded blastocysts after exposure >80%.
    • Bottles and screw caps are M.E.A. tested.
    • Store at 2 to 30°C and avoid temperatures above or below these values. Under these conditions ThermoBlast has a shelf-life of 12 months from production. The expiry date is shown on both bottles and cartons.
    • Open and close bottles under aseptic conditions. After opening store at 2 to 8°C when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
    • No antibiotics, unstable additives or preservatives have been added by the manufacturer to ThermoBlast.
    • Use aseptic procedures at all times.
    • Do not use any vial or solution that shows evidence of particulate matter or cloudiness.
    • Closed Vitrification system is mandatory in USA.
    • Federal Law (USA) restricts this device to sale by or on the order of a physician.
    • Please check for regulatory compliance governing the use of ART products in your country.
  • Article No. / Name / Volume
    TBK-010 / ThermoBlast Kit / 4×10 mL (Not available in Europe)

  • SDS – View PDF

    Product insert (latest version) – View PDF

    For other languages, contact Nidacon

  • Prediction of live birth in frozen-thawed single blastocyst transfer cycles by pre-freeze and post-thaw morphology.

    Ahlström A1, Westin C, Wikland M, Hardarson T., (2013)
    Human Reproduction, Vol.28, No.5 pp. 1199–1209

    Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique.

    Lane M et al, (1999)
    Fertility and Sterility., Vol 72, No 6, pp1073-1078

    Vitrification of human blastocysts using cryoloops: Clinical outcome of 223 cycles.

    Mukaida T et al. (2003)
    Hum Reprod., Vol. 18, No. 2, pp384-391

    Perinatal outcome of blastocyst transfer with vitrification using cryoloop: A 4 year follow-up study.

    Takahashi K et al. (2005)
    Fertil Steril., Vol. 84, No. 1, 88-92

    Obstetric outcome of children born after transfer of vitrified blastocysts

    Wikland M et al. (2010).
    Human Reproduction, Vol.00, No.0 pp. 1–9

    Vitrification and warming human blastocysts by use of a laser to artificially induce blastocyst collapse prior to vitrification.

    T. Hardarson et al. (2006)
    Acta Obstet Gynecol Scand. 86 pp119-120

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