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An alternative method, the UniLayer filtration of semen, of preparing human sperm for IUI
Many clinicians in small clinics or private practices are looking for a safe and effective method of preparing sperm for intra-uterine insemination. They are unsure of their ability to layer a density gradient correctly to achieve optimal results. However, they also know that a “swim up” does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation.
Now there is an alternative method of preparing human sperm for IUI – the PureSperm SpeediKit. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes.
It can be extremely difficult to obtain good yields of motile sperm from highly viscous semen samples. Nidacon has developed a method that could be helpful to you in the lab. You simply add PureSperm Buffer to the ejaculate, incubate at 37°C for 15-30 minutes and the sample is ready to use. This will give you a much higher proportion of motile sperm.
For sperm preparation from a viscous semen sample:
1. Bring all solutions to room temperature.
2. Measure the volume of the semen sample.
3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample
(e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
4. Incubate at 37°C for 15-30 minutes.
5. Mix using a pipette.
6. Ready for sperm preparation on a density gradient.
Article No. / Name / Volume
PSSK-010 / PureSperm SpeediKit / 10 patient preps