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Speedikit

An alternative method, the UniLayer filtration of semen, of preparing human sperm for IUI 

Many clinicians in small clinics or private practices are looking for a safe and effective method of preparing sperm for intra-uterine insemination. They are unsure of their ability to layer a density gradient correctly to achieve optimal results. However, they also know that a “swim up” does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation.

  • Now there is an alternative method of preparing human sperm for IUI – the PureSperm SpeediKit. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes.

    • Sodium chloride
    • Potassium chloride
    • Magnesium sulphate
    • Potassium dihydrogen phosphate
    • Sodium bicarbonate
    • EDTA
    • hSA (human Serum Albumin)
    • Sodium citrate
    • Glucose
    • Calcium lactate
    • Sodium pyruvate
    • HEPES
    • Purified water
    • Silane-coated silica

    Performance Characteristics

    • pH of PureSperm UniLayer: 7.4 – 7.8.
    • pH of PureSperm Wash: 7.3 – 8.5.
    • Osmolality of PureSperm UniLayer (mOsm/kg H2O): 300 – 330.
    • Osmolality of PureSperm Wash (mOsm/kg H2O): 290 – 300.
    • Endotoxin levels (EU/mL): <1.0.
    • Contents are tested by human sperm survival only.
    • Tubes and stoppers are M.E.A. tested.
    • Store at 2 to 27ºC and avoid temperatures above or below these values. Under these conditions the contents of the kit have a shelf-life of minimum 12 months from production. The expiry date is shown on both tubes and cartons. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
    • No antibiotics, unstable additives or preservatives have been added by the manufacturer to PureSperm SpeediKit.
    • When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.
    • Use aseptic procedures at all times.
    • If available, use sealed buckets during centrifugation to avoid creation of aerosols.
    • Clean accidental spills using a dampened cloth or paper. PureSperm SpeediKit causes floors and benches to be extremely slippery.
    • PureSperm SpeediKit does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
    • Do not use any solution which shows evidence of bacterial contamination.
    • Do not use contents if tamper-evident seal is broken.
    • Federal Law (USA) restricts this device to sale by or on the order of a physician.
    • Please check for regulatory compliance governing the use of ART products in your country.
  • It can be extremely difficult to obtain good yields of motile sperm from highly viscous semen samples. Nidacon has developed a method that could be helpful to you in the lab. You simply add PureSperm Buffer to the ejaculate, incubate at 37°C for 15-30 minutes and the sample is ready to use. This will give you a much higher proportion of motile sperm.

    Protocol: 
    For sperm preparation from a viscous semen sample:

    1. Bring all solutions to room temperature.
    2. Measure the volume of the semen sample.
    3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample
    (e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
    4. Incubate at 37°C for 15-30 minutes.
    5. Mix using a pipette.
    6. Ready for sperm preparation on a density gradient.

  • Article No. / Name / Volume

    PSSK-010 / PureSperm SpeediKit / 10 patient preps (Not available in Europe)

  • SDS – View PDF

    Product insert (latest version) – View PDF

    For other languages, contact Nidacon

    Leaflet – View PDF

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