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Ready-diluted to save gradient preparation time.
All the advantages of a PureSperm 100 gradient, plus ready-diluted to save preparation time. PureSperm 40, PureSperm 80 in both 100mL & 20mL and PureSperm 90 in 100mL, are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from the semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential. Watch video tutorial here.
It can be extremely difficult to obtain a high yield of motile sperm from highly viscous, semen samples. Nidacon has developed a method that can be helpful in solving this problem in the lab. You simply add PureSperm Buffer to the ejaculate, incubate for 15-30 minutes at 37°C and the sample is ready to use with reduced viscosity. Importantly, this will give you a much higher yield of motile sperm.
For sperm preparation from a viscous semen sample:
1. Bring all solutions to room temperature.
2. Measure the volume of the semen sample.
3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample
(e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
4. Incubate at 37°C for 15-30 minutes.
5. Mix using a pipette.
6. Ready for sperm preparation on a density gradient.
Article No. / Name / Volume
PSK-020 / PureSperm 40/80 / 2 × 20 mL
PS40-100 / PureSperm 40 / 100 mL
PS80-100 / PureSperm 80 / 100 mL
PS90-100 / PureSperm 90 / 100 mL
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