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Ready-diluted to save gradient preparation time.
All the advantages of a PureSperm 100 gradient, plus ready-diluted to save preparation time. PureSperm 40, PureSperm 80 in both 100mL & 20mL and PureSperm 90 in 100mL, are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from the semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential. Watch video tutorial here.
It can be extremely difficult to obtain a high yield of motile sperm from highly viscous, semen samples. Nidacon has developed a method that can be helpful in solving this problem in the lab. You simply add PureSperm Buffer to the ejaculate, incubate for 15-30 minutes at 37°C and the sample is ready to use with reduced viscosity. Importantly, this will give you a much higher yield of motile sperm.
For sperm preparation from a viscous semen sample:
1. Bring all solutions to room temperature (17 to 27°C).
2. Measure the volume of the semen sample.
3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample
(e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).
4. Incubate at 37°C for 15-30 minutes.
5. Mix using a pipette.
6. Ready for sperm preparation on a density gradient.
Article No. / Name / Volume
PSK-020 / PureSperm 40/80 / 2 × 20 mL
PS40-100 / PureSperm 40 / 100 mL
PS80-100 / PureSperm 80 / 100 mL
PS90-100 / PureSperm 90 / 100 mL
Stefania Luppi1, Monica Martinelli1, Elisa Giacomini2, Elena Giolo1, Gabriella Zito2, Rodolfo C Garcia3† and Giuseppe Ricci12*†
Reproductive Biology and Endocrinology 2015, 13:36
Iraj Amiri, Marzieh Ghorbani, Safora Heshmati
Journal of Clinical and Diagnostic Research. 2012 November, Vol-6(9): 1451-1453
Charoenchai Chiamchanya, Nattpawit Kaewnoonual, Pachara Visutakul, Sirikul Manochantr and Jirattikan Chaiya.
Asian J Androl. 2010 Mar;12:271-277.
Sakkas D, Manicardi GC, Tomlinson M, Mandrioli M, Bizzaro D, Bianchi PG, Bianchi
U.Hum Reprod. 2000 May;15(5):1112-6.
Henkel R, Kierspel E, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, Schill WB, Kruger TF.
Reprod Biomed Online. 2003 Oct-Nov;7(4):477-84.
P Ranganathan, A Agarwal
Fertil Steril, 2001
Allamaneni SS, Agarwal A, Rama S, Ranganathan P, Sharma RK.
Asian J Androl. 2005 Mar;7(1):86-92.
C.M. Nicholson L. Abramsson, S.E. Holm and E. Bjurulf
Human Reproduction, Vol. 15, No. 3, 662-666, March 2000
M. Tomlinson et al
Abstract Andrology in the nineties 1999
Agarwal, A. and Ranganathan, P.ESHRE 2001
J. A. Roudebush, et al.
Supplement of the 27th Annual Meeting of American Society of Andrology, 81 Seattle, USA (March/April 2002)
Nathalie Mousset-Siméon, Nathalie Rives, Lydie Masse, Florence Chevallier and Bertrand Mace
Journal of Andrology, Vol. 25, No. 6, November/December 2004