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Optimised one step staining technique for assessment of sperm motility and morphology.
The Sperm VitalStain from Nidacon contains both eosin and nigrosine. Sperm VitalStain™ is an optimised staining technique for assessment of sperm vitality and morphology, which is one of the basic tools used in semen analysis.
Sperm VitalStain™ is a one-step vital staining technique containing eosin and nigrosin. The concept of using eosin uptake to mark dead cells, and nigrosin background stain, increasing the contrast between stained cells and otherwise bright background, is well known and widely used.
The technique is based on the principle that dead cells (i.e. those with damaged plasma membrane) will take up the eosin and stain red. Nigrosin is used as a counterstain to facilitate visualization of the unstained (white) live cells.
Store at 10-40°C. Under these conditions Sperm VitalStain has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons.
Article No. / Name / Volume
SVS-010 / Sperm VitalStain / 2 × 10mL
Why the WHO Recommendations for Eosin-Nigrosin Staining Techniques for Human Sperm Vitality Assessment Must Change
Lars Björndahl, Inget Söserlund, Sofia Johansson, Majid Mohammadieh, Mohammad Reza Pourian and Ulrik Kvist.
Journal of Andrology, Vol. 25, No. 5, September/October 2004