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BoviDilute

Maximizes sperm survival during density gradient centrifugation.

Dilute BoviPure with BoviDilute to desired concentrations to form either a one-layer- or a two-layer gradient for density centrifugation. The pellet retrieved after centrifugation contains only normal, purified spermatozoa. BoviDilute can also be used to extend bovine semen prior to centrifugation with BoviPure.

    • An isotonic salt solution.
    • Unopened bottles have a shelf-life of 24 months.
    • pH of 7.4-7.8 at room temperature and at 37°C.
    • Osmolality (mOsm/kg H2O): 300-310.
    • Sterilised by filtration.
    • Content tested by sperm survival only.
    • Packaging is made up of borosilicate glass bottles, silicone stoppers and tamper-evident seals.
    • Bottles and stoppers have been M.E.A. tested.
      • Sodium chloride
      • Sodium citrate
      • Potassium chloride
      • HEPES
      • Calcium lactate
      • EDTA
      • Sodium pyruvate
      • Glucose
      • Purified water
    • Store unopened bottles at 2 to 40°C and avoid temperatures above or below these values. Under these conditions BoviDilute has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons.
    • Open and close bottles under aseptic conditions. After opening, store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
    • No antibiotics, unstable additives or preservatives have been added by the manufacturer to BoviDilute.
    • Store unopened bottles at 2 – 40°C, avoid temperatures above or below these values. Under these conditions BoviDilute has a shelf life of 12 months. The expiry date is shown on the labels.
    • Open and close bottles under aseptic conditions. After opening, store the bottles at 2 – 8°C.
    • No antibiotics, unstable additives or preservatives have been added to BoviDilute.
    • To ensure best possible results use aseptic conditions at all times.
    • When washing the semen, follow the instructions given in the product insert to avoid inadvertent contamination.
    • When centrifuging, use sealed buckets or centrifuge tubes to avoid the creation of aerosols.
    • BoviDilute does not represent any fire or combustion hazard. A material safety data sheet is available from the manufacturer or distributor.
  • Article No. / Name / Volume
    BD-100 / BoviDilute / 100 mL

  • A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF.

    Samardzija M, Karadjole M, Matkovic M, Cergolj M, Getz I, Dobranic T, Tomaskovic A, Petric J, Surina J, Grizelj J, Karadjole T.
    Anim Reprod Sci. 2006 Feb;91(3-4):237-47
    Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
    “Our results indicate that both protocols gave suitable sperm for IVF. This finding and the similarity between those two density gradient preparations suggests that BoviPure is a good alternative for sperm separation in bovine IVF.”

    Effects of bovine spermatozoa preparation on embryonic development in vitro.

    Samardzija M et al
    Reprod Biol Endocrinol. 2006 Nov 13;4 (1):58 17101040
    “So, we concluded that BoviPure® could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos”.

    Retained Functional Integrity of Bull Spermatozoa after Double Freezing and Thawing Using PureSperm® Density Gradient Centrifugation.

    WMC Maxwell 1 , I Parrilla 2 , I Caballero 2 , E Garcia 2 , J Roca 2 , EA Martinez 2 , JM Vazquez 2 and D Rath 3
    Reproduction in Domestic Animals, 2007 Oct, Volume 42 Issue 5, Pages 489 – 494
    “These results demonstrate that bull semen can be frozen and thawed, followed by a second freeze-thawing cycle of a population of spermatozoa selected by PureSperm®, with retained motility and functional integrity. This points to the possibility of using double frozen spermatozoa in bovine artificial insemination programmes and to the potential benefits of PureSperm® density gradient centrifugation for the application of cryopreserved bull spermatozoa to other biotechnological procedures such as flow cytometric sex sorting followed by re-freezing and thawing.”

    Develompent of Procedures for Sex-Sorting Frozen-Thawed Bovine Spermatozoa.

    Underwood et al
    Reproduction in Domestic Animals, 2009 Jun;44(3):460-6. Epub 2008 Nov 2
    “In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.

    BoviPure Pro

    Effect of Density Gradient Centrifugation with Trypsin on the Fertilizing Capability of Bovine Sperm .

    B. A. Blevins, M. de la Rey and N. M. Loskutoff
    Reproduction, Fertility and Development 2008;20(7): 784 – 8
    “In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.

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