FREEZING PROTOCOL FOR SPERMCRYOPROTEC
COULD WE MAKE IT EASIER FOR THE EMBRYOLOGIST, WHILE MAINTAINING THE HIGH SURVIVAL RATE?
1. The first is the incubation time in the fridge after loading the straws.
The new range is now 10-60 minutes instead of earlier 30-60 minutes. If you look at the results when incubating for 60 minutes compared to 10, you can see a slight increase when incubated for 60 minutes. The big difference is however between not incubating and incubating for 10 minutes.
2. The second change is the time on the CryoFloater in LN2
The new recommendation is 10-30 minutes instead of only 30 minutes. We have again performed a lot of tests, both at Nidacon and with the help of Akademiska Hospital in Uppsala, Sweden. If you measure the temperature on the floater, it does go down quite fast and 10 minutes is more than enough but, in order to make it more flexible, we now recommend the range 10-30. No difference in result with regards to survival rate has been found in our tests between 10 and 30 minutes but it will give you the chance to take the coffee you so desperately need!