Cryopreservation of Mammalian Gametes and Embryos
Methods and Protocols
Nagy, Zsolt Peter, Varghese, Alex C., Agarwal, Ashok (Eds.)
This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations.
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Appendix G: Vitrification of blastocysts using VitriBlast and ThermoBlast:
Anna Niläng, Thorir Hardarsson, Ann-Sofie Forsberg, Tetsunori Mukaida, and Paul V. Holmes.
This appendix describes the vitrification of blastocysts using VitriBlast and ThermoBlast, from Nidacon. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.