Select sperm with longer telomeres and eliminate sperm with dna fragmentation
Selection of functional sperm with less DNA damage and longer telomeres should be prerequisites for achieving optimal outcomes for ART.
DNA fragmentation contributes to the failure of assisted reproductive technologies, and can ultimately lead to failed fertilization and increases the risk of abnormal embryo development, while telomere shortness is associated with males who are infertile (Zhao, F. et al.)
Tips and Tricks:
Density Gradient Centrifugation is the optimal sperm preparation method in order to avoid DNA fragmentation and to select the sperm with the longer telomeres.
Simple: ready-to-use density gradient solutions, 40/80 and 90%, make work in the lab easier and they minimize the risk for mistakes.
Flexible: a 40/80 or a 40/90 combination. Both are two-layer systems for density gradients, the 40/90 combination giving higher sperm motility, while the 40/80 gives higher sperm yield
REF: Zhao, F. et al. Semen preparation methods and sperm telomere length: density gradient centrifugation versus the swim-up procedure.
Image credit: Genome Research Limited