• When should PureSperm® Wash be used?

- We recommend PureSperm® Wash for washing the sperm pellet after centrifugation using a PureSperm® density gradient. The ionic balance of PureSperm® Wash lies between that of the gradient and commonly used fertilisation media and, therefore, will not cause premature hyperactivation of the sperm. PureSperm® Wash is also ready-to-use and contains an appropriate level of human serum albumin.

• What are the constituents of PureSperm® Wash?

- PureSperm® Wash is an isotonic (290-300 mOsm/kg H2O), HEPES-buffered salt solution containing glucose, EDTA and human serum albumin.

• Is there any protein in PureSperm® Wash?

- Research in our laboratory showed that human serum albumin must be added to the PureSperm® Wash to prevent sperm sticking to plastic-ware and microscope slides.

• Are antibiotics included in PureSperm® Wash?

- No, PureSperm® Wash does not contain antibiotics, for the following reasons: 1. Antibiotics commonly used in cell culture media are toxic to sperm. 2. The gradient will remove most, if not all, of the bacterial contamination present in the ejaculate, provided that the retrieval of the sperm pellet is carried out according to the instructions given in the package insert.

• Should PureSperm® Wash be refrigerated?

- PureSperm® Wash is supplied as a sterile solution which does not need refrigerated storage in the unopened bottle. However, after opening under sterile conditions, PureSperm® Wash should be kept at 2-8°C.

Human Products