• How do I use PureSperm® Buffer ?

PureSperm® Buffer is used to dilute PureSperm®.to obtain the layers of different densities required for a discontinuous density gradient. PureSperm® Buffer has been carefully formulated to give optimal results when used according to the manufacturer´s instructions. It is ready-to-use, no additives being required.

• Why do I need to use PureSperm® Buffer to dilute PureSperm®? I have previously used a commercially available washing medium from another company.

A comparison has shown that you will obtain a better yield of motile sperm from using PureSperm® Buffer for diluting PureSperm® than non-optimised products. commercially available culture media, washing media or salt solutions such as Earles´Balanced Salt Solution have not been optimised for this purpose. Furthermore, salt solutions such as Earles, requires other ingredients to be added before use, thus increasing the cost.

• Why do I need to buy a separate product, such as PureSperm® Buffer to dilute PureSperm®? Could I use PureSperm® Wash instead?

Yes, you can use PureSperm® Wash to dilute PureSperm®. However, a comparison in our laboratories has shown that you will obtain a better yield of motile sperm from using PureSperm® Buffer which has been optimised for this purpose.

• Should I equilibrate PureSperm® Buffer before use?

If the bottle of PureSperm® Buffer has been opened previously and then stored in the refrigerator, it should be brought up to room temperature before use to avoid cold-shocking the sperm. It is not necessary to equilibrate PureSperm® Buffer in the CO2 incubator before use

• Are there any antibiotics in PureSperm® Buffer ?

No, PureSperm® Buffer does not contain antibiotics. Many commonly-used antibiotics are toxic to sperm. Furthermore, antibiotics such as penicillin, have a very short half-life in solution. When used according to the manufacturer´s instructions, the PureSperm® density gradient will enable an uncontaminated sperm preparation to be obtained from the semen sample.

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