The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies.
Sakkas D, Manicardi GC, Tomlinson M, Mandrioli M, Bizzaro D, Bianchi PG, Bianchi
U.Hum Reprod. 2000 May;15(5):1112-6.
DNA fragmentation of spermatozoa and assisted reproduction technology.
Henkel R, Kierspel E, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, Schill WB, Kruger TF.
Reprod Biomed Online. 2003 Oct-Nov;7(4):477-84.
Recovery and survival of sperm is higher with PureSperm density gradient than swim-up in neat and cryopreserved-thawed semen specimen.
P Ranganathan, A Agarwal
Fertil Steril, 2001
Comparative study on density gradients and swim-up preparation techniques utilizing neat and cryopreserved spermatozoa.
Allamaneni SS, Agarwal A, Rama S, Ranganathan P, Sharma RK.
Asian J Androl. 2005 Mar;7(1):86-92.
Bacterial contamination and sperm recovery after semen preparation by density gradient centrifugation using silane-coated silica particles at different g forces
C.M. Nicholson L. Abramsson, S.E. Holm and E. Bjurulf
Human Reproduction, Vol. 15, No. 3, 662-666, March 2000
The use of PureSperm, mini-Percoll and swim-up preparation techniques to separate spermatozoa with chromatin and nuclear DNA anomalies.
M. Tomlinson et al
Abstract Andrology in the nineties 1999
Higher rates of recovery with PureSperm density gradient compared to ISolate
Agarwal, A. and Ranganathan, P.ESHRE 2001
A comparison of two density gradient preparations for sperm separation for medically assisted conception: Effect on recovery, clean-up, motility, and motion parameter.
J. A. Roudebush, et al.
Supplement of the 27th Annual Meeting of American Society of Andrology, 81 Seattle, USA (March/April 2002)
Comparison of Six Density Gradient Media for Selection of Cryopreserved Donor Spermatozoa
NATHALIE MOUSSET-SIMÉON, NATHALIE RIVES, LYDIE MASSE, FLORENCE CHEVALLIER AND BERTRAND MACE
Journal of Andrology, Vol. 25, No. 6, November/December 2004
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