Summary

All the advantages of a PureSperm®100 gradient PLUS ready-diluted to save preparation time!

PureSperm® 40 and PureSperm®80 are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.

PureSperm® 40 and PureSperm®80 maintain pH between 7.4 - 7.8 at room temperature and at 37°C, providing suitable conditions for good sperm motility, survival time, and fertilization potential.

The preparation contains glucose as a usable energy substrate for normal cell metabolism and sperm function.

The gradient enables motile sperm to be separated from extraneous cells and seminal plasma. In addition, damage to sperm DNA and structural molecules caused by reactive oxygen species (ROS) is minimized by the effective removal of immature sperm and lymphocytes. Bacteria, epithelial cells, and cell debris are also separated from the motile sperm, which can be difficult to achieve in polysucrose based gradient preparations.

Components

Silane-coated silica
Potassium ions
Sodium ions
Purified water
HEPES
EDTA
Glucose
Citrate
Lactate
Pyruvate

Performance Characteristics

pH: 7.4 - 7.8
Osmolality (mOsm/kg H2O) : 300 - 310
Endotoxin transfer during treatment : <1.0 EU/mL
Sperm survival 18 hours after density gradient separation: >70%
Contents are tested by human sperm survival only
Bottles and stoppers are 2-cell M.E.A. tested

Storage and Stability

Store unopened bottles at 2 to 40ºC and avoid temperatures above or below these values. Under these conditions PureSperm® 40 and PureSperm® 80 has a shelf-life of 24 months. The expiry date is shown on both bottles and cartons.

Open and close bottles under aseptic conditions. After opening, store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.

No antibiotics, unstable additives or preservatives have been added by the manufacturer to PureSperm® 40 and PureSperm® 80.

Precautions and Warnings

- When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination

- Use aseptic procedures at all times.

- If available, use sealed buckets during centrifugation to avoid creation of aerosols

- Clean accidental spills using a dampened cloth or paper. PureSperm® 40/80 causes floors and benches to be extremely slippery

- PureSperm® 40/80 does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer(see nidacon.com)

- Do not use any solution which shows evidence of bacterial contamination

- Do not use contents if tamper-evident seal is broken

- Federal Law (USA) restricts this device to sale by or on the order of a physician

- Please check for regulatory compliance governing the use of ART products in your country

Ordering Information

Article No. / Name / Volume
PSK-020 / PureSperm® 40/80 / 2 × 20 mL
PS40-100 / PureSperm® 40 / 100 mL
PS80-100 / PureSperm® 80 / 100 mL

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