• What should I use for diluting PureSperm100?

PureSpermBuffer is the best media to use for dilution of PureSperm100. It has been optimised and ionically balanced to match PureSperm

• Is there any human serum albumin in PureSperm 100?

- Research in our laboratory showed that the yield of motile sperm recovered from the gradient was approximately the same, regardless of whether or not the gradient layers contained human serum albumin. Therefore, we have not included any protein in PureSperm 100. The colloid in these products eliminates aggregation of sperm and reduces their sticking to the centrifuge tube. However, human serum albumin must be added to the "wash" solution, as provided in PureSperm Wash.

Are antibiotics included in PureSperm 100?

- No, PureSperm 100 does not contain antibiotics, for the following reasons: 1. The gradient will remove most, if not all, of the bacterial contamination present in the ejaculate, provided that the retrieval of the sperm pellet is carried out according to the instructions given in the package insert. 2. Antibiotics commonly used in cell culture media are toxic to sperm.

• What should be used for washing the sperm pellet after the PureSperm 100 gradient centrifugation?

- We recommend PureSperm Wash for washing the sperm pellet after gradient centrifugation. The ionic balance of PureSperm Wash lies between that of the gradient and commonly used fertilisation media and, therefore, will not cause premature hyperactivation of the sperm. PureSperm Wash is also ready-to-use and contains an appropriate level of human serum albumin.

Why do I need to buy a separate product, such as PureSperm® Buffer to dilute PureSperm®100? Could I use PureSperm® Wash instead?

- Yes, you can use PureSperm® Wash to dilute PureSperm®. However, a comparison in our laboratories has shown that you will obtain a better yield of motile sperm from using PureSperm® Buffer which has been optimised for this purpose.

Do we need to incubate PS100 and PS40/80 in 6% CO2 , 37°C before use?

- The most important to avoid is temperature fluctuations for the sperm. The ejaculate is approximately around room temperature or slightly higher. Therefore we recommend equilibrating PureSperm to room temperature, not more. The preparation is also done at room temperature and therefore the temperature is still maintained.

Human Products