Im12MorfoStainSummary

The Nidacon Sperm MorfoStain™ is a classical Romanovsky type stain. It is a one step stain optimised for assessment of sperm morphology, which is one of the basic tools used in semen analysis. The technique is based on the principle that the sperm will be stained in a darker colour (blue) and the backround will be lighter and therefore the shape, size and integrity of the sperm can easily be seen in oil immersion under a microscope.

DottedDivide

Components

Methanol
Eosin Y
Methylene B
May Grunwald
Giemsa
Azur B

DottedDivide1

Storage and Stability

Store at 10-22oC. Under these conditions Sperm MorfoStain™ has a shelf-life of 12 months. The expiry date is shown on both bottles.

DottedDivide2

Precautions and Warnings

Sperm MofoStain™is highly flammable.

Inhalation: Remove from exposure. If discomfort persists. obtain medical attention

Skin contact: Wash of with abundance of water. Remove contaminated clothing

Eye contact: Flush thouroughly with physiological saline for 15 min. Obtain medical attention

Ingestion: Rinse mouth with water and give water to drink. Obtain medical attention

MSDS is available from the manufacturer (see nidacon.com)

DottedDivide3

Reagents and Equipment

• Coplin jar or similar
• Glas microscope slide holder
• Microscope slides
• Light microscope (400-1000x magnification)
• Pipette

ContinuousDivide