A comparison of BoviPure and Percoll on bull sperm separation protocols for IVF.
Samardzija M, Karadjole M, Matkovic M, Cergolj M, Getz I, Dobranic T, Tomaskovic A, Petric J, Surina J, Grizelj J, Karadjole T.
Anim Reprod Sci. 2006 Feb;91(3-4):237-47
Clinic for Obstetrics and Reproduction, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia
“Our results indicate that both protocols gave suitable sperm for IVF. This finding and the similarity between those two density gradient preparations suggests that BoviPure is a good alternative for sperm separation in bovine IVF”
Effects of bovine spermatozoa preparation on embryonic development in vitro.
Samardzija M et al
Reprod Biol Endocrinol. 2006 Nov 13;4 (1):58 17101040
“So, we concluded that BoviPure® could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos”.
Retained Functional Integrity of Bull Spermatozoa after Double Freezing and Thawing Using PureSperm® Density Gradient Centrifugation.
WMC Maxwell 1 , I Parrilla 2 , I Caballero 2 , E Garcia 2 , J Roca 2 , EA Martinez 2 , JM Vazquez 2 and D Rath 3
Reproduction in Domestic Animals, 2007 Oct, Volume 42 Issue 5, Pages 489 - 494
“These results demonstrate that bull semen can be frozen and thawed, followed by a second freeze-thawing cycle of a population of spermatozoa selected by PureSperm®, with retained motility and functional integrity. This points to the possibility of using double frozen spermatozoa in bovine artificial insemination programmes and to the potential benefits of PureSperm® density gradient centrifugation for the application of cryopreserved bull spermatozoa to other biotechnological procedures such as flow cytometric sex sorting followed by re-freezing and thawing.”
Develompent of Procedures for Sex-Sorting Frozen-Thawed Bovine Spermatozoa.
Underwood et al
Reproduction in Domestic Animals, 2009 Jun;44(3):460-6. Epub 2008 Nov 2
“In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.
BoviPure Pro
Effect of Density Gradient Centrifugation with Trypsin on the Fertilizing Capability of Bovine Sperm .
B. A. Blevins, M. de la Rey and N. M. Loskutoff
Reproduction, Fertility and Development 2008;20(7): 784 – 8
“In summary, BoviPure Pro sperm treatment before AI significantly increases fertilization rates and can result in as much as an 18.5% increase in transferable quality embryos as compared to standard methods”.
