Intended use
BoviWash™ is a medium for diluting sperm prior to gradient treatment and for washing the sperm pellet after density gradient separation. It minimises sudden pH and osmotic changes during the transition from semen sample to fertilisation medium. It also maximises the yield of motile sperm and improves the fertilisation potential.
Specifications
- An isotonic salt solution
- Unopened bottles have a shelf-life of 12 months
- pH of 7.25 - 7.35 at room temperature and at 37°C
- Sterilised by filtration
- Content tested by sperm survival only
- Bottles are made of borosilicate glass and silicone stoppers with tamper-evident seals.
- Bottles and stoppers have been 2-cell M.E.A. tested
Components
- Potassium ions
- Calcium ions
- Sodium ions
- Chloride ions
- Magnesium
- Sulphate
- Phosphate
- Carbonate
- Pyruvate
- Lactate
- Glucose
- HEPES
- EDTA
- Purified water
Storage and stability
Store unopened bottles at 2 - 40°C, avoid temperatures above or below these values. Under these conditions BoviWash™ have a shelf life of 12 months. The expiry date is shown on the labels.
Open and close bottles under aseptic conditions. After opening, store the bottles at 2 - 8°C.
No antibiotics, unstable additives or preservatives have been added to BoviWash™
Precautions and Warnings
- To ensure best possible results use aseptic conditions at all times.
- When washing the semen, follow the instructions given in the insert to avoid inadvertent contamination.
- When centrifuging use sealed, buckets or centrifuge tubes, to avoid the creation of aerosols.
- BoviWash™ does not represent any fire or combustion hazard. A material safety data sheet is available from the manufacturer or distributor.
Other Bovine Products
Other products in the bovine series include the BoviPure™ Top and Bottom Layer. This is density gradient system for purification of bovine sperm through centrifugation. BoviExtend™ is a medium used for extending Bovine sperm prior to purification through a density gradient.
