EquiPure™ Bottom and Top Layer is a density gradient system used for separating and purifying Equine spermatozoa. This is a standard procedure used in the purification of sperm. EquiPure™ Bottom Layer is ready to use in conjunction with EquiPure™ Top Layer to compose the layers in the gradient. Together they build two density interfaces at which the sperm will be filtered. The pellet that is retrieved after centrifugation contains only normal purified spermatozoa.
Specifications -Colloidal silical particles coated with silane, in an isotonic salt solution. -Unopened bottles have a shelf-life of 2 years -pH of 7.4 – 7.8 at room temperature and at 37°C -Low endotoxin levels (<5.00 EU/mL) -Terminally sterilised by autoclaving -Bottles are made of borosilicate glass and silicone stoppers with tamper-evident seals. -Bottles and stoppers have been 2-cell M.E.A. tested
Components -Silane-coated silica -Potassium ions -Calcium ions -Sodium ions -Chloride ions -Pyruvate -Lactate -Citrate -Glucose -HEPES -EDTA -Water quality - WFI
Storage and stability Store unopened bottles at 2 - 40°C, avoid temperatures above or below these values. Under these conditions EquiPure™ Bottom and Top Layers have a shelf life of 2 years. The expiry date is shown the labels. Open and close bottles under aseptic conditions. After opening, store the bottles at 2 - 8°C. No antibiotics, unstable additives or preservatives have been added to EquiPure™ Bottom or Top Layer.
Precautions and Warnings -When retrieving the sperm pellets, follow the instructions given in the insert to avoid inadvertent contamination. -To ensure best possible results use aseptic conditions at all times. -Use sealed, buckets or centrifuge tubes during centrifugation to avoid the creation of aerosols. -Clean accidental spills with dampened cloth or paper. EquiPure™ Bottom and Top Layers cause floors and bench tops to be slippery. - EquiPure™ Bottom and Top Layers do not represent any fire or combustion hazard. A material safety data sheet is available from the manufacturer or distributor.
For veterinary use only
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Intended use
